Plant
Biotechnology
A Practical Approach
H. S. Chawla
978-1-57808-296-4; 2003; 312 pages, pb; US $ 44.20
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Now
Plant biotechnology has now become firmly
entrenched as a branch of science. Biotechnology courses have been included
in the basic course curriculum of schools and universities. To understand
biotechnology, it is essential that students learn elementary laboratory
protocols on plant tissue culture and recombinant DNA technology.
This
practical laboratory manual has been designed to familiarise one with such
protocols. The book describes simple plant tissue culture protocols that can
be performed on easily available plant material, and also those meant for
specific materials. Worksheets have been provided to record the results of
the experiments. Exercises have been linked to one another, for a better
understanding of the rationale for performing the exercise.
Contents:
Part I: Basics. Introduction
. Laboratory
Organization
. Sterilization Techniques
. Nutrition
Medium
. Choice of Explant
Part II: Plant Tissue
Culture. Seed Culture
.
Micropropagation-Meristem Culture
. Micropropagation-Axillary
Bud Proliferation
. Micropropagation-Adventitious Regeneration
.
Micropropagation-Organogensis
. Micropropagation-Embryogensis
.
Cell Suspension
. Secondary Metabolite Production in a Cell
Suspension Culture
. Anther Culture
. Protoplast
Isolation and Fusion
Part III: Biotechnology.
Sds-page Electrophoresis of Proteins
. Isolation of DNA from
Plant Tissues
. Isolation of Plasmid DNA
.
Restriction Enzyme Digestion of DNA
. Agarose Gel
Electrophoresis
. Preparation of Competent Cells, Transformation
of E. Coli with Plasmid DNA and Ligation of Insert DNA to a Vector
.
Agrobacterium Mediated Gene Transfer
. Biolistic Method of
Transformation in Plants
. In Vitro Amplification of DNA by
Pcr-detection of Transgenes
. Rapd Analysis
.
Microsatellite Marker Analysis
. Southern Blotting
.
Southern Hybridization
Appendix. Metric
Prefixes
. Greek Alphabets
. Molarity Concentrations
of some Commonly used Plant Growth Regulators
. Composition of
some Plant Tissue Culture Media (values expressed as mg per liter)
.
Mineral Composition of Vacin and Went (1946) Medium for Orchid Culture
.
Mineral Composition of some Media Commonly used in Anther/Microscope Culture
.
Kao and Michyaluk (1975) 8p Media for Protoplast Culture Medium PC I of
Frearson et al. (1973)
. K3 Medium for Protoplast Culture (Nagy
and Maliga, 1976)
. Nagata and Takebe (1971) Protoplast Culture
Medium
. White's Minerals used for Protoplast Culture (White,
P.R., 1963)
. Nucleic Acid Conversion Factors
.
Agarose Gel Percentages for Resolution of Linear DNA
.
Polyacrylamide Gel Percentages for Resolution of DNA
. Trouble
Shooting Guide during Electrophoresis
. SOB Medium Composition
.
Phenol Equilibration
. YEM Medium Composition
.
Construction of Dendogram
