Introduction to Plant Biotechnology
Second Edition
H.S. Chawla
ISBN 978-1-57808-228-5; 2002; 562 Pages; US $ 48.00      Buy Now


In recent years, plant biotechnology has emerged as an exciting area of research by creating unprecedented opportunities for the manipulation of biological systems of plants. To understand biotechnology, it is essential to know the basic aspects of gene and its organisation in the genome of plant cells. Plant tissue culture and recombinant DNA technology constitutes an important aspect of plant biotechnology. This textbook is meant for students to grasp all aspect of the subject.

Review of First Edition
"... an excellent introduction ... appropriate for upper-division undergraduate and graduate students, faculty, and many practicing plant scientists who graduated before the genetic evolution."


Contents:
PLANT TISSUE CULTURE
. Introduction
. Laboratory Organization
. Nutrition Medium
. Sterilization Techniques
. Types of Culture
. Micropropagation
. Cell Suspension and Secondary Metabolites
. In vitro Production of Haploids
. Protoplast Isolation and Fusion
. Somaclonal Variation
. Germplasm Storage and Cryopreservation

GENETIC MATERIAL AND ITS ORGANIZATION
. Genetic Material
. Organization of DNA and Gene Expression

RECOMBINANT DNA TECHNOLOGY
. Basic Techinques.
. Gene Cloning-Cuttting and Joining DNA Molecules.
. Gene-cloning Vectors
. Gene Cloning- cDNA and Genomic Cloning and Analysis of Cloned DNA Sequences.
. Polymerase Chain Reaction
. In Vitro Mutagenesis
. Transposon Genetic Elements and Gene Tagging
. Gene Isolation
. Molecular Markers and Marker Assisted Selection
. Gene Transfer Methods
. Transgenics in Crop Improvement
. Genomics, DNA Chips, and Microarrays
. Intellectual Property Rights